IMPACT (Intein Mediated Purification with an Affinity Chitin-binding Tag) is a novel protein purification system which utilizes the inducible self-cleavage activity of a protein splicing element (termed intein) to separate the target protein from the affinity tag. It distinguishes itself from all other purification systems by its ability to purify, in a single chromatographic step, a native recombinant protein without the use of a protease. A target protein is fused to a tag consisting of the intein and the chitin binding domain which allows affinity purification of the fusion protein on a chitin column.
In the presence of thiols such as DTT or 2-mercaptoethanesulfonic acid (MESNA), the intein undergoes specific self-cleavage which releases the target protein from the chitin-bound intein tag resulting in a single-column purification of the target protein.
The IMPACT Kit contains expression vectors which allow fusion of the cleavable intein tag to either the C-terminus or N-terminus of the target protein. This flexibility in fusion protein construction maximizes the probability of successful expression and purification of a target protein.
Available IMPACT products:
| PRODUCT | Catalog # | SIZE | INFO | |
|---|---|---|---|---|
| IMPACT Kit | E6901S | 1 Kit |  |
 |
| Chitin Resin | S6651S | 20 ml | |
|
| Chitin Resin | S6651L | 100 ml | |
|
| Chitin Magnetic Beads | E8036S | 5 ml | |
|
| pTWIN1 Vector DNA | N6951S | 10 µg | |
|
| pTXB1 Vector DNA | N6707S | 10 µg | |
|
| pTYB21 Vector | N6709S | 10 µg | |
|
| Anti-CBD Monoclonal Antibody | E8034S | 0,05 ml | |
|
As of 03.01.2024
Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.
