With the phi29-XT DNA polymerase and the phi29-XT RCA kit
Rolling circle amplification (RCA) is an isothermal amplification method to continuously amplify circular DNA by generating long, repetitive copies of the circular sequence.
The phi29-XT DNA Polymerase is an engineered polymerase with improved thermostability and sensitivity generating higher yield in a shorter reaction time than wild-type phi29 DNA Polymerase.
- High yield in a short reaction time
- Amplify from 1 fg circular DNA input
- Flexible protocol for various input materials
- Enables multiple downstream applications without further processing steps
- Kit includes dNTPs and exonuclease-resistant random primers
The phi29-XT RCA Kit includes everything needed for sensitive and robust rolling circle amplification The kit features the phi29-XT DNA Polymerase. Also included are dNTPs and exonuclease-resistant random primers (containing phosphorothioate bonds) to universally amplify circular DNA sequences. Input material can be purified circular DNA (single or double-stranded) or direct from liquid media culture, agar plate colonies, and glycerol stocks without the need for DNA extraction. RCA products can be used directly in downstream applications such as DNA sequencing, cell-free DNA enrichment, cell-free protein expression, and DNA biosensors.
The phi29-XT RCA Kit
View the Enhanced Rolling Circle Amplification Performance with a Newly Engineered phi29 DNA Polymerase NEB TV webinar on demand.
Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.
