Optimized to address the challenges of FFPE DNA samples
Archiving of clinical materials as Formalin-Fixed, Paraffin-Embedded (FFPE) samples is a common practice. However, the methods used for fixation and storage severely damage the nucleic acids from these samples. Additionally, FFPE DNA samples are often available in only very small amounts (e.g., a microns-thick tissue section or a region of interest from a laser-capture microdissection). As a result, it can be challenging to construct high quality libraries in sufficient quantity to achieve good sequence data at the desired depth of coverage. FFPE DNA repair is an essential step towards obtaining high-quality libraries from formalin fixed cells and tissues.
- Repair of FFPE-induced DNA damage
- Broad input range (5-250 ng)
- Increased library yield
- Greater sensitivity of somatic variant calling
- Automation-friendly workflow
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NEBNext FFPE DNA Repair V2 Module
The NEBNext FFPE DNA Repair V2 Module improves FFPE DNA repair through an optimized combination of enzymes, designed to be used upstream of NGS library prep for the repair of several of the most common types of damage caused by FFPE treatment. The latest version, “V2”, offers an even higher repair efficiency and a more streamlined workflow.
Figure 1:
Workflow demonstrating the use of the NEBNext FFPE DNA Repair v2 Module with the NEBNext Ultra II DNA Library Prep Kit for Illumina.
(Click on image to enlarge)
Figure 2:
The NEBNext FFPE DNA Repair v2 Module enables robust library preparation from a broad range of FFPE DNA sample qualities.
(Click on image to enlarge)
NEBNext UltraShear FFPE DNA Library Prep Kit
The NEBNext UltraShear FFPE DNA Library Prep Kit is the newest option for high-quality library preparation from FFPE-treated samples. This kit incorporates enzymatic fragmentation of FFPE DNA using NEBNext UltraShear, a new enzyme mix optimized for use with FFPE DNA that increases library yields and quality, while improving scalability and ease of use. The kit also repairs damage to DNA caused by the FFPE process and includes reagents and a protocol optimized for library prep of FFPE DNA. The workflow is streamlined and user-friendly, with minimal hands-on time, and the kit improves yields and quality of libraries from a range of FFPE DNA sample input (5-250 ng) and quality.
Figure 3:
NEBNext UltraShear FFPE DNA Library Prep Kit workflow.
(Click on image to enlarge)
NEBNext FFPE DNA Library Prep Kit
For FFPE samples for which enzymatic fragmentation is not desired, the NEBNext FFPE DNA Library Prep Kit is designed for pre-sheared FFPE DNA and improves yields and quality of libraries from a range of sample inputs (5-250 ng) and quality.
Figure 4:
NEBNext FFPE DNA Library Prep Kit workflow.
(Click on image to enlarge)
Product table
| PRODUCT | Prod. # | SIZE | PRICE | ||
|---|---|---|---|---|---|
| NEBNext® FFPE DNA Library Prep Kit | M6650S | 24 rxns |  |
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| M6650L | 96 rxns | |
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| NEBNext® UltraShear FFPE DNA Library Prep Kit | E6655S | 24 rxns | |
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| E6655L | 96 rxns | |
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| NEBNext® FFPE DNA Repair v2 Module | E7360S | 24 rxns | |
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| E7360L | 96 rxns | |
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| NEBNext® FFPE DNA Repair Mix | M6630S | 24 rxns | |
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| M6630L | 96 rxns | |
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As of: 07.06.2024
NEBNext products are available at a permanent low price that can not be further discounted.
Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.
