Exonucleases and endonucleases are used in many of today’s molecular biology workflows and applications.
Did you know that NEB offers the largest supply of these important tools, and has a team of experts studying the function and optimization of these enzymes?
We also offer several helpful tools to help you find the best enzyme to facilitate your work.
Key Examples:
Thermolabile USER II Enzyme
Thermolabile USER (Uracil-Specific Excision Reagent) II Enzyme generates a single nucleotide gap at the location of a uracil residue. It can be 100% inactivated at temperatures >65°C, streamlining workflows and enabling DNA to be used directly in downstream applications.
T7 Endonuclease I
T7 Endonuclease I recognizes and cleaves non-perfectly matched DNA, cruciform DNA structures,
Holliday structures or junctions, heteroduplex DNA and more slowly, nicked double-stranded DNA. The cleavage site is at the first, second or third phosphodiester bond that is 5´ to the mismatch. The protein is the product of T7 gene 3.
Thermostable FEN1
Thermostable Flap Endonuclease 1, FEN1, catalyzes the cleavage of 5´ DNA flaps from branched double stranded DNA substrates, creating a 5´ phosphate terminus. FEN1 products can be ligated by DNA ligase to create double-stranded DNA. In vivo, FEN1 is an essential component of the Okazaki fragment maturation pathway, and also plays a role in base excision repair.
Lambda Exonuklease
Lambda Exonuclease catalyzes the removal of nucleotides from linear or nicked double-stranded DNA in the 5´ to 3´ direction. This enzyme is highly processive. The preferred substrate is 5´-phosphorylated double-stranded DNA, although non-phosphorylated substrates are degraded at a greatly reduced rate.
Thermolabile Exonuclease I
Thermolabile Exonuclease I catalyzes the removal of nucleotides from linear single-stranded DNA in
the 3´ to 5´ direction. Unlike Exonuclease I (NEB #M0293), Thermolabile Exonuclease I can be heat
inactivated at 80°C in one minute.
Exonuclease V (RecBCD)
Exonuclease V (RecBCD) is a DNA specific exonuclease that also acts as an endonuclease on singlestranded DNA. It initiates at both 5′ and 3′ termini of linear double-stranded DNA and cleaves linear
double-stranded DNA in both the 3′ to 5′ and 5′ to 3′ directions. It requires ATP in the reaction.
Free Download:
Feature Article – The effect of nucleic acid modifications on digestion by DNA exonucleases
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