Accelerate your cloning experiments with reagents from NEB
While cloning has typically been a time-consuming process in the past, advances in both technologies and reagents have enabled the process to take much less time. Race to NEB for the highest quality products that save valuable time!
Click on the “vial” icons to learn more!
Fast cloning reagents from NEB:
SEAMLESS CLONING
NEBuilder HiFi DNA Assembly Master Mix, NEBuilder HiFi DNA Assembly Cloning Kit & NEBuilder HiFi DNA Assembly Bundle for Large Fragments
Rapid, seamless assembly/cloning of multiple fragments in as little as 15 minute
NEBridge Golden Gate Assembly Kit (BsaI-HF®v2)
Efficient, ordered assembly of multiple fragments in a single reaction
DIGESTION
High-Fidelity (HF) restriction enzymes
HF enzymes offer reduced star activity, rapid digestion and 100% activity in CutSmart® Buffer
Time-Saver restriction enzymes
>195 enzymes are Time-Saver qualified and will digest DNA in 5-15 minutes; digest overnight with no unwanted star activity
END MODIFICATION
Complete dephosphorylation in 10 minutes with rapid, irreversible heat inactivation
Blunt restriction enzyme-digested DNA in less than 30 minutes at room temperature
AMPLIFICATION
Q5 High-Fidelity DNA Polymerases
With the lowest error rate of all known commercially available PCR polymerases, Q5 enables extension times as low as 10 seconds per kb on templates up to 20 kb
PURIFICATION
Purify high-quality DNA in minutes (5 minutes for the PCR & DNA Cleanup Kit!). Designed with sustainability in mind, Monarch kits use less plastic and responsibly-sourced packaging
LIGATION
Instant Sticky-end Ligase Master Mix
Specially formulated to instantly ligate 2-4 basepair overhangs! Just add DNA, flick the tube, and ligation occurs instantly! No need for incubation.
Specially formulated to ligate single-base and blunt ends in just 15 minutes
Ligates both cohesive and blunt ends in just 5 minutes at room temperature
TRANSFORMATION
NEB Turbo Competent E. coli (High-Efficiency)
Generate colonies in as little as 6.5 hours with a 5-minute transformation protocol
Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.
