Quickly & Easily: Purify high quality DNA from PCR and other enzymatic reactions
The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times.
- Elute in as little as 5 μl for highly concentrated DNA, with yields up to 5 μg
- No need to monitor buffer pH
- Protocol modification for oligonucleotide cleanup is provided, allowing purification of ssDNA, oligonucleotide and other small DNA fragments
Protocol Video:Monarch PCR & DNA Cleanup
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Designed with sustainability in mind
We achieve this by using particularly thin-walled columns and bottles. In this way we reduce the use of plastic and thus the amount of waste in the laboratory. In addition, all components of the packaging are made of recycled material.
Unique Column Design
NEB Monarch’s unique column design and membrane assembly allows high-quality, highly-concentrated nucleic acid purification with low elution volume, for downstream applications. The column is designed and made with significantly less plastic for a reduced environmental impact.
The Monarch Spin PCR & DNA Cleanup Kit (5 μg) effectively cleans up oligonucleotide DNA.
Synthesized ssDNA (≥ 16 nt) and dsDNA (≥12 bp) oligonucleotides were purified following the oligonucleotide cleanup protocol using 1 μg of oligonucleotides of varying lengths (8–25 nt/bp). DNA was eluted in 20 μl of Monarch Buffer EY. Concentrations of DNA were measured, and percent recovery calculations are based on the eluted DNA concentration and elution volume used. The minimum sized ss- and ds- oligonucleotides that can be used are marked with a star (*).
Tips for using the Monarch PCR & DNA Cleanup Kit
Optimize your DNA isolation from PCR and other enzymatic reactions with our quick tips for using the Monarch PCR & DNA Cleanup Kit.
Video: PCR & DNA Cleanup Tips
Monarch Spin PCR & DNA Cleanup Products:
| PRODUCT | Art. # | SIZE | INFO | |
|---|---|---|---|---|
| Monarch Spin PCR & DNA Cleanup Kit (5ug) | T1130S/L | 50 / 250 preps |  |
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| Monarch Spin Columns S1A | T2037L | 100 preps | |
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| Monarch Spin Collection Tubes | T2118L | 100 preps | |
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As of: 22.08.2024
Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.
