The new standard in NGS Library Prep for Illumina Platforms.
Get even more from less with NEBNext Ultra II!
Are you challenged with trying to obtain higher library yields using ever-decreasing input amounts? Each component in the NEBNext Ultra II DNA Library Prep Kit from NEB® has been reformulated, resulting in a several-fold increase in library yield with as little as 500 picograms of input DNA. These advances deliver unprecedented performance, while enabling lower inputs and fewer PCR cycles.
Request a free NEBNext Ultra II Library Prep sample!
Use our interactive overview of the NEBNext Ultra II workflow to identify the perfect kit for your sequencing project:
Video: Ultra II Library Prep Protocol
- Get more of what you need, with the highest library yields
- Generate high quality libraries even when you have only limited amounts of DNA, with inputs as low as 500 pg
- Prepare libraries from ALL of your samples, including GC-rich targets and FFPE DNA samples
- Improve yields for target enrichment applications
- Save time with streamlined workflows, reduced hands-on time, and automation compatibility, and enjoy the flexibility of kit or module format products
Now also available as digital interactive NEBNext Ultra II DNA protocol!
See what others are saying about NEBNext Ultra II:
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„The possibility to use the new NEBNext Ultra II DNA kit has provided us with a critical opportunity to process challenging low-input genomic samples. These samples otherwise didn't yield libraries of adequate complexity, required for exploring their genomes comprehensively. Using the NEBNext Ultra II DNA Kit has alleviated this problem and enabled us to deliver high quality, high content sequencing data that are relevant for our users.“ Dr. Vladimir Benes, Head of GeneCore, EMBL Heidelberg |
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„The protocol is very easy to follow and is a step up from the standard NEB Ultra Kit. Using as little as 500 pg of cell-free DNA input, we were able to generate libraries that were successfully used for subsequent exome capture.“ Dr. Christopher Smith, Post-Doctoral Research Associate at the Cancer Research UK - Cambridge Institute (CRUK-CI) |
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Brochure:
NEBNext for Illumina – NGS Sample Preparation
Technical Note:
“Improved library preparation with the
NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®“
For more technical details click here.
Available NEBNext Ultra II DNA products
| PRODUCT | Prod. # | SIZE | INFO | |
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| NEBNext Ultra II DNA PCR-Free Library Prep Kit | E7410S | 24 rxns |  |
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| NEBNext Ultra II DNA PCR-Free Library Prep Kit | E7410L | 96 rxns | |
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| NEBNext Ultra II DNA PCR-Free Library Kit w Beads | E7415S | 24 rxns | |
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| NEBNext Ultra II DNA PCR-Free Library Kit w Beads | E7415L | 96 rxns | |
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| NEBNext® Ultra II DNA PCR-Free Library Kit for Illumina® with Beads | E7415S | 24 rxns | |
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| E7415L | 96 rxns | |
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| NEBNext® Ultra II DNA Library Prep Kit for Illumina® | E7645S | 24 rxns | |
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| E7645L | 96 rxns | |
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| NEBNext® Ultra™ II DNA Library Kit with Purification Beads | E7103S | 24 rxns | |
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| E7103L | 96 rxns | |
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| NEBNext® Ultra II End Repair/dA-Tailing Module | E7546S | 24 rxns | |
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| E7546L | 96 rxns | |
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| NEBNext® Ultra II Ligation Module | E7595S | 24 rxns | |
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| E7595L | 96 rxns | |
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| NEBNext® Ultra II Q5® Master Mix | M0544S | 50 rxns (50 µl/vol) | |
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| M0544L | 250 rxns (50 µl/vol) | |
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As of: 01.01.2024
NEBNext products are available at a permanent low price that can not be further discounted.
Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.
