NEBNext Immune-Sequencing Kit

Generate full-length immune gene repertoires of B and T cells from human or mouse cells

With the NEBNext Immune Sequencing Kits (available for mouse and human), sequence the full-length immune gene repertoires of B cells and T cells. Profile somatic mutations across all relevant contexts (e.g., V, D, and J segments and isotypes IgM, IgD, IgG, IgA, and IgE) with improved sequence accuracy. Characterize BCR light, BCR heavy, TCRα, TCRβ, TCRγ and TCRδ chains.

This kit includes UMIs for source-molecule identification. Additionally, analyze data using a bioinformatic workflow based on the open-source pRESTO toolkit (tutorial).