Q5 High Fidelity DNA Polymerase

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Five Quality Features of Q5

Q1 Fidelity

The highest fidelity amplification available (~280X higher than Taq, ~7x higher than Phusion Polymerase)”Ultra-Fidelity” (~280X higher than Taq, ~7x higher than Phusion Polymerase)

Q2 Robustness

High specificity and yield with minimal optimization

Q3 Coverage

Superior performance for a broad range of amplicons (from high AT to high GC)

Q4 Speed

Short extension times

Q5 Amplicon length

Robust amplifications up to 20 kb for simple templates, and 10 kb for complex

Video: Important tips for Q5 Polymerase

Feature Article:

„Polymerase Fidelity –
What is it and what does it mean for your PCR?“

Polymerase-Fidelity--What-is-it-and-what-does-it-mean-for-your-PCR
Download PDF

Comparison of High-Fidelity Polymerases

1 We continue to investigate improved assays to characterize Q5’s very low error rate to ensure thatwe present the most accurate fidelity data possible (Potapov, V. and Ong, J.L. (2017) PLoS ONE.12(1): e0169774.).
2 PCR-based mutation screening in lacZ (NEB), lacI (Agilent) or rpsL (Life)
3 Template not specified.
4 Simple templates include plasmid, viral and E. coli genomic DNA. Complex templates include plant, human and other mammalian genomic DNA.
5 Values provided by individual manufacturers.

Amplification Performance Across a Wide Range of Genomic Targets

PCR was performed with a variety of amplicons, with GC content ranging from high AT to high GC, with Q5 and several other commercially available polymerases. All polymerases were cycled according to manufacturer’s recommendations, including use of GC Buffers and enhancers when recommended. Yield and purity of reaction products were quantitated and represented, as shown in the figure key, by dot color and size. A large dark green dot represents the most successful performance. Q5 provides superior performance across the range of GC content.

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PCR

Recommended products:

  • Q5 High-Fidelity DNAPolymerase (NEB #M0491)
  • Q5 Hot Start High-Fidelity DNA Polymerase (NEB #M0493)
  • Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515)
  • Q5 High-Fidelity 2X Master Mix (NEB #M0492)
  • Q5 Hot Start High-Fidelity 2X Master Mix (NEB #M0494)
  • Q5 High-Fidelity PCR Kit (NEB #E0555)
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NGS LIBRARY AMPLIFICATION

Recommended products:

  • NEBNext Ultra II Q5 Master Mix (NEB #M0544)
  • NEBNext Q5U Master Mix (NEB #M0597)
Icon_ArticViral

ARTIC VIRAL SEQUENCING

Recommended products:

One-Step

  • LunaScript Multiplex One-Step RT-PCR Kit (NEB #E1555)

Two-Step

  • LunaScript RT SuperMix (NEB #M3010)
  • Q5 Hot Start High- Fidelity 2X Master Mix (NEB #M0494)
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DIRECT SAMPLE AMPLIFICATION

Recommended products:

  • Q5 Blood Direct 2X Master Mix (NEB #M0500)
Icon_Mutagenese

MUTAGENESIS

Recommended products:

  • Q5 Site-Directed Mutagenesis Kit (NEB #E0554)
  • Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) (NEB #E0552)

Available Q5 Polymerase Products

PRODUCT Prod. # Size INFO
Q5 High-Fidelity DNA Polymerase M0491S 100 units In den Shop wechseln Produktinformationen
M0491L 500 units In den Shop wechseln Produktinformationen
Q5 High-Fidelity 2X Master Mix M0492S 100 rxns (50 µl/vol) In den Shop wechseln Produktinformationen
M0492L 500 rxns (50 µl/vol) In den Shop wechseln Produktinformationen
Q5 Hot Start High-Fidelity DNA Polymerase M0493S 100 units In den Shop wechseln Produktinformationen
M0493L 500 units In den Shop wechseln Produktinformationen
Q5 Hot Start High-Fidelity 2X Master Mix M0494S 100 rxns (2×1.25ml) In den Shop wechseln Produktinformationen
M0494L 500 rxns (10×1.25ml) In den Shop wechseln Produktinformationen
M0494X 500 rxns (1×12.5ml) In den Shop wechseln Produktinformationen
Q5U Hot Start High-FidelityDNA Polymerase M0515S 100 units In den Shop wechseln Produktinformationen
M0515L 500 units In den Shop wechseln Produktinformationen
Auch erhältlich als Q5 High-Fidelity PCR Kit:
Q5 High-Fidelity PCR Kit E0555S 50 rxns In den Shop wechseln Produktinformationen
E0555L 200 rxns In den Shop wechseln Produktinformationen
Auch erhältlich als Next-Gen-Seq optimierte Formulierung:
NEBNext Ultra II Q5 Master Mix M0544S 50 rxns (50 µl vol) In den Shop wechseln Produktinformationen
M0544L 250 rxns (50 µl vol) In den Shop wechseln Produktinformationen
M0544X 500 rxns (50 µl vol) In den Shop wechseln Produktinformationen
NEBNext High-Fidelity 2X PCR Master Mix M0541S 50 rxns (50 µl/vol) In den Shop wechseln Produktinformationen
M0541L 250 rxns (50 µl/vol) In den Shop wechseln Produktinformationen
NEBNext Q5 Hot Start HiFi PCR Master Mix M0543S 50 rxns (50 µl vol) In den Shop wechseln Produktinformationen
M0543L 250 rxns (50 µl vol) In den Shop wechseln Produktinformationen
NEBNext Q5U Master Mix M0597S 50 rxns (50 µl/vol) In den Shop wechseln Produktinformationen
M0597L 250 rxns (50 µl/vol) In den Shop wechseln Produktinformationen
oder für “ARTIC” ähnliche Workflows:
One-Step:
LunaScript Multiplex One-Step RT-PCR Kit
E1555S 50 rxns In den Shop wechseln Produktinformationen
E1555L 250 rxns In den Shop wechseln Produktinformationen
Two-Step:
LunaScript RT SuperMix
M3010L 100 rxns In den Shop wechseln Produktinformationen
M3010X 500 rxns In den Shop wechseln Produktinformationen
M3010E 2500 rxns In den Shop wechseln Produktinformationen
Auch erhältlich als Direct Sample Amplification Formulierung:
Q5 Blood Direct 2X Master Mix M0500S 100 rxns (1x1ml) In den Shop wechseln Produktinformationen
M0500L 500 rxns (5x1ml) In den Shop wechseln Produktinformationen
Auch erhältlich für Mutagenesis:
Q5 Site-Directed Mutagenesis Kit E0554S 10 rxns In den Shop wechseln Produktinformationen
Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) E0552S 10 rxns In den Shop wechseln Produktinformationen
Passend dazu:
Deoxynucleotide Solutions, Mix N0447S 8 µmol of each In den Shop wechseln Produktinformationen
N0447L 40 µmol of each In den Shop wechseln Produktinformationen

As of: 01.01.2024

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