Five Quality Features of Q5
Q1 Fidelity
The highest fidelity amplification available (~280X higher than Taq, ~7x higher than Phusion Polymerase)”Ultra-Fidelity” (~280X higher than Taq, ~7x higher than Phusion Polymerase)
Q2 Robustness
High specificity and yield with minimal optimization
Q3 Coverage
Superior performance for a broad range of amplicons (from high AT to high GC)
Q4 Speed
Short extension times
Q5 Amplicon length
Robust amplifications up to 20 kb for simple templates, and 10 kb for complex
Video: Important tips for Q5 Polymerase
Feature Article:
„Polymerase Fidelity –
What is it and what does it mean for your PCR?“
Comparison of High-Fidelity Polymerases
1 We continue to investigate improved assays to characterize Q5’s very low error rate to ensure thatwe present the most accurate fidelity data possible (Potapov, V. and Ong, J.L. (2017) PLoS ONE.12(1): e0169774.).
2 PCR-based mutation screening in lacZ (NEB), lacI (Agilent) or rpsL (Life)
3 Template not specified.
4 Simple templates include plasmid, viral and E. coli genomic DNA. Complex templates include plant, human and other mammalian genomic DNA.
5 Values provided by individual manufacturers.
Amplification Performance Across a Wide Range of Genomic Targets
PCR was performed with a variety of amplicons, with GC content ranging from high AT to high GC, with Q5 and several other commercially available polymerases. All polymerases were cycled according to manufacturer’s recommendations, including use of GC Buffers and enhancers when recommended. Yield and purity of reaction products were quantitated and represented, as shown in the figure key, by dot color and size. A large dark green dot represents the most successful performance. Q5 provides superior performance across the range of GC content.
PCR
Recommended products:
Available Q5 Polymerase Products
| PRODUCT | Prod. # | Size | INFO | ||
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| Q5 High-Fidelity DNA Polymerase | M0491S | 100 units |  |
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| M0491L | 500 units | |
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| Q5 High-Fidelity 2X Master Mix | M0492S | 100 rxns (50 µl/vol) | |
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| M0492L | 500 rxns (50 µl/vol) | |
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| Q5 Hot Start High-Fidelity DNA Polymerase | M0493S | 100 units | |
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| M0493L | 500 units | |
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| Q5 Hot Start High-Fidelity 2X Master Mix | M0494S | 100 rxns (2×1.25ml) | |
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| M0494L | 500 rxns (10×1.25ml) | |
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| M0494X | 500 rxns (1×12.5ml) | |
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| Q5U Hot Start High-FidelityDNA Polymerase | M0515S | 100 units | |
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| M0515L | 500 units | |
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| Auch erhältlich als Q5 High-Fidelity PCR Kit: | |||||
| Q5 High-Fidelity PCR Kit | E0555S | 50 rxns | |
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| E0555L | 200 rxns | |
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| Auch erhältlich als Next-Gen-Seq optimierte Formulierung: | |||||
| NEBNext Ultra II Q5 Master Mix | M0544S | 50 rxns (50 µl vol) | |
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| M0544L | 250 rxns (50 µl vol) | |
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| M0544X | 500 rxns (50 µl vol) | |
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| NEBNext High-Fidelity 2X PCR Master Mix | M0541S | 50 rxns (50 µl/vol) | |
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| M0541L | 250 rxns (50 µl/vol) | |
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| NEBNext Q5 Hot Start HiFi PCR Master Mix | M0543S | 50 rxns (50 µl vol) | |
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| M0543L | 250 rxns (50 µl vol) | |
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| NEBNext Q5U Master Mix | M0597S | 50 rxns (50 µl/vol) | |
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| M0597L | 250 rxns (50 µl/vol) | |
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| oder für “ARTIC” ähnliche Workflows: | |||||
| One-Step: LunaScript Multiplex One-Step RT-PCR Kit |
E1555S | 50 rxns | |
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| E1555L | 250 rxns | |
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| Two-Step: LunaScript RT SuperMix |
M3010L | 100 rxns | |
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| M3010X | 500 rxns | |
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| M3010E | 2500 rxns | |
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| Auch erhältlich als Direct Sample Amplification Formulierung: | |||||
| Q5 Blood Direct 2X Master Mix | M0500S | 100 rxns (1x1ml) | |
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| M0500L | 500 rxns (5x1ml) | |
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| Auch erhältlich für Mutagenesis: | |||||
| Q5 Site-Directed Mutagenesis Kit | E0554S | 10 rxns | |
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| Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) | E0552S | 10 rxns | |
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| Passend dazu: | |||||
| Deoxynucleotide Solutions, Mix | N0447S | 8 µmol of each | |
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| N0447L | 40 µmol of each | |
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As of: 01.01.2024
Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.
