Reliable isothermal amplification of whole genomes
The phi29-XT WGA Kit is optimized for sensitive, fast, and robust whole genome amplification (WGA), an isothermal method for amplification of an entire genome, starting from femtogram quantities of DNA and resulting in microgram quantities of amplified products. The kit features phi29-XT DNA Polymerase, an engineered polymerase with improved thermostability, sensitivity, and capable of generating a higher yield in a shorter reaction time than wild-type phi29 DNA Polymerase. Also included are exonuclease-resistant random primers (containing phosphorothioate bonds) to universally amplify any DNA sequence, neutralization buffer, and dNTPs. Input material can be purified genomic DNA, cells, or microbiomes. WGA enables several downstream applications, including single cell whole genome sequencing (WGS), genomic DNA enrichment, and environmental DNA (eDNA) enrichment and sequencing.
- Powered by phi29-XT DNA Polymerase, an engineered polymerase with improved thermostability and sensitivity
- High sensitivity: amplify from as little as 10 fg of input DNA
- Flexible protocol offers compatibility with different types of sample input material
- Simple workflow produces high yield in a short reaction time (less than 2 hours)
- Whole genome representation for human and microbial DNA
- Enables multiple downstream applications without further processing steps
Available Products
| PRODUCT | Art.Nr.: | SIZE | Info | |
|---|---|---|---|---|
| phi29-XT WGA Kit | E1604S | 25 rxns |  |
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| phi29-XT WGA Kit | E1604L | 100 rxns | |
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As of: 17.09.2024
Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.
