„Cloning 2.0“ – DNA Assembly and cloning products for synthetic biology
The field of synthetic biology is steadily gaining popularity, not only because of the international iGEM competition which was supported by NEB recently.
Due to the high interest in synthetic biology, new cloning and gene editing methods were developed. These methods allow to create large, artificial DNA structures through re-organisation, editing or assembly of genes.
NEB offers a variety of innovative „Cloning 2.0“ products to simplify your daily cloning workflows.
Order your favorite Cloning 2.0 product now!

PCR with highest accuracy:
Use the “error-free” Q5 high-fidelity DNA polymerase – over 280 times more accurate than Taq and particularly reliable on all templates (AT-rich, GC-rich, up to 20 kb).
Engineering Genes with NEBs EnGen Products:
highly efficient EnGen Cas9 NLS, EnGen sgRNA Synthesis Kit, EnGen Mutation Detection Kit and more.
NEBuilder HiFi DNA Assembly
Simple and restriction enzyme-free assembly and cloning of DNA fragment in less than 1 hour.
Golden Gate Assembly
Effective and seamless BsaI-based ligation of DNA fragments.
BioBrick Assembly
The BioBrick Assembly Kit provides a streamlined method for assembly of BioBrick parts into multi-component genetic systems.
Cloning Strains
Try our „ready to use“ strains for your cloning needs. Convenient formats and excellent transformation rates simplify your daily lab routine.
Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.