DNA Fragmentierung kombiniert mit Library Prep – NEBNext Ultra II FS DNA

„The Wellcome Trust Sanger Institute currently processes thousands of DNA samples each month via its core DNA sequencing library construction pipelines. However, a recent requirement to generate high quality whole genome and targeted sequencing data from biopsy material led us to develop and implement a novel workflow enabled by NEBNext Ultra II FS. Our new automated workflow, coupled with the high efficiency of the NEBNext Ultra II FS reagent, is allowing us to routinely generate deep sequence data from as few as 100-1000 human cells.“

Dr. Peter Ellis Senior Staff Scientist, R&D Sequencing, Wellcome Trust Sanger Institute

„NEBNext Ultra II FS performance is exceptional. The possibility to start with higher DNA concentrations allows for less consumption of reagents for initial QC. Obtaining the desired fragment length and less hands-on time are also key factors when preparing genomic libraries and NEBNext Ultra II FS can provide that. The use of fewer PCR cycles also decreases the bias associated with the PCR step.“

João Anes, Ph,D., NGS specialist in Food Safety, UCD-Centre for Food Safety, Dublin, Ireland

„I don’t know of any other NGS library prep kit that works so well with small genomes/DNA fragments e.g. bacterial, phage genomes, plasmids, PCR products.“

Anton Bryksin, Ph.D., Director, Molecular Evolution Core, Parker H. Petit Institute for Bioengineering and Bioscience

„In our core, we process large number of samples for genomics applications. Even with the high-throughput Covaris® model, the sample shearing is still a bottle neck for us. The NEBNext DNA Ultra II FS DNA kit is such a wonderful product. First of all, it increased our throughput dramatically for DNA sample shearing. The kit combines the shearing, end-repair, dA-tailing, and linker-ligation in a couple simple steps without the need of purification in between. The whole process has been reduced from two days to a few hours. Second, we love the low input option. Because the kit eliminates the need for purification for each of the intermediate steps, it reduces a lot of sample loss during the purification steps, which enables us to use much less starting materials and saves tons on purification beads. Third, we are amazed by the randomness of the enzymatic shearing. We did not observe any significant difference from mechanical shearing. We have used this kit successfully with many large projects including high-throughput genomic screening assays.“

Scientist, Cancer research institute

„This kit is fantastic! I was able to create a high quality genomic library from 200 pg of DNA using the NEB Ultra II FS Kit. I had a non-model organism, limited material, GC bias and no experience with genomic library prep. The enzymatic fragmentation meant I didn’t lose any precious material in shearing and I was more than happy with the yield.“

Dr. Ashlie Hartigan, Kings College London