Klonierung & Synthetische Biologie
Tools
- NEBBiocalculator
- NEBCloner
- NEBuilder
- NEB Golden Gate Assembly Tool
- Double Digest Finder
- NEB Cutter
- DNA Sequences and Maps Tool
- Enzyme Finder
Selection Tools
- Synthetic Biology/DNA Assembly Selection Chart
- DNA Ligase Selection Chart
- Isoschizomere der Restriktionsenzyme
Troubleshooting Guides
- Troubleshooting Guide for Cloning
- Restriction Enzyme Troubleshooting Guide
- Troubleshooting Guide for Ligases
- Troubleshooting Guide for End Modification
Hinweise und Tipps
- Restriction Enzyme Tips
- Optimizing Restriction Endonuclease Reactions
- Restriction enzymes requiring multi-sites for efficient cleavage
- NEBuffer Activity/Performance Chart with Restriction Enzymes
- Activity of Restriction Enzymes in a Q5®, Taq or Phusion PCR Mix
- Cloning Plasmids and DNAs Overview
- Compatible Cohesive Ends and Generation of New Restriction Sites
- Dam-Dcm and CpG Methylation
- Frequencies of Restriction Sites
- Recleavable Blunt Ends
- Recleavable Filled-in 5′ Overhangs
- Cleavage of Supercoiled DNA
- Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
- Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
- Restriction Endonucleases – Survival in a Reaction
- Traditional Cloning Quick Guide
- Tips for Maximizing Ligation Efficiencies
- End-Modification Tips
- Cleavage Close to the End of DNA Fragments
- Effect of various temperatures on Nicking Endonucleases
- Application Notes
- Improved methods for site-directed mutagenesis using Gibson Assembly® Master Mix
- Joining of Difficult to Ligate dsDNA Fragments with Blunt/TA Ligase Master Mix
Video Tutorials
RNA Biologie
Hinweise und Tipps
- RNA Polymerase Selection Chart
- RNA Ligase Selection Chart
- cDNA Synthesis Selection Chart
- Recommended HiScribe RNA Synthesis Kits by Application
Guides
Video Tutorials
Glykobiologie
Tools
Hinweise und Tipps
FAQs
Application Notes
- α1-3,6 Galactosidase Application Note
- Enzymatic removal of N- and O-glycans using PNGase F or the Protein Deglycosylation Mix P0704
- O-Glycosidase Application Note 1
Video Tutorials
Epigenetik
Troubleshooting Guides
Application Notes
Video Tutorials
DNA / RNA Leitern
Selection Tools
PCR DNA Amplifikation
Tools
Selection Tools
Troubleshooting Guides
Hinweise und Tipps
- Guidelines for PCR Optimization with Taq DNA Polymerase
- Guidelines for PCR Optimization with OneTaq® and OneTaq® Hot Start DNA Polymerases
- Guidelines for PCR Optimization with Thermophilic DNA Polymerases
FAQs
Application Notes
Video Tutorials
Next Generation Sequencing
Tools
Selection Tools
Hinweise und Tipps
Kompetente Zellen
Selection Tools
- Characteristics of Select E.coli Strains
- Competent Cell Selection Guide
- Competitor Cross-Reference Tool
Troubleshooting Guides
Hinweise und Tipps
- Genetic Markers
- Making your own electrocompetent cells
- Making Unmethylated (Dam- Dcm-) DNA
- Restriction of Foreign DNA by E. coli K-12
- Additional E. coli Strain Genotypes
- McrA, McrBC and EcoKI Strain Phenotypes
Video Tutorials
Proteinexpression
Antikörper-freie Zellfärbung (SNAP Tag)
Selection Tools
Troubleshooting Guides
FAQs
Application Notes
- Labeling of Escherichia coli Expressed SNAP-tag® Fusion Proteins
- Labeling and Imaging of Cell Surface Receptors Mediated by SNAP-tag®
- Simultaneous Fluorescent Labeling of Proteins in Living Cells
- Simultaneous Labeling of Two Proteins in Live Cells
- SNAPf based pulse labeling for analysis of protein turnover in living cells
Video Tutorials
Video Library: NEBTV Episoden, Podcast und Webinare
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